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Unlike MSCs or ASCs, SVF contains a more heterogeneous cell population that includes endothelial, pericytic, progenitor and hematopoietic cells, all of which perform distinct biological functions. However, SVF therapy presents many unmet challenges. The mechanism proposed in the majority of these clinical studies is the paracrine effect, rather than the differentiation paradigm. Nevertheless, this subset of cells and their therapeutic properties are currently being investigated in hundreds of clinical trials, according to. Comparison between MSCs and ASCs remains a controversial topic. To date, it has not been fully elucidated whether ASCs or mesenchymal stem/stromal cells (MSCs) from other tissues share the same therapeutic potential. Recent findings indicate that adipose tissue, more specifically its stromal vascular fraction (SVF) component, is a rich and accessible source of cells for regenerative medicine therapeutic approaches by means of paracrine factors, the existence of progenitor cells and more notably, the isolation of a subset of cells named adipose stem/stromal cells (ASCs). The safety and regenerative capacity of so-called “adult stem cells” has been the source of much discussion in the scientific community. Keywords: Mesenchymal stem cell Autologous stem cells Adipose Cartilage Noteworthy, the histological analysis indicated that there was no cartilage growth, suggesting an alternative therapeutic mechanism to cartilage regeneration. Finally, both cell populations were able to ameliorate disease progression, as confirmed by the increase in movement of treated groups compared to injured groups. PCR analysis showed no significant numbers of cells outside the joint 1-hour post injection, moreover, no engraftment or abnormal growth in other organs 60-days post injection. However, T cell activation with anti-CD3 or anti-CD3+ anti-CD28, while leading to expected high levels of IFN-γ, did not lead to significant levels of TGF-β.
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ELISAs showed that following LPS activation in SVF, there were low levels of TNF-α and high levels of IL-10 secreted. However, there was a negative correlation between donor BMI and MSC frequency within the SVF. Flow cytometry showed that neither age nor BMI affects the frequency of progenitor cells-like (CD31+CD34+), immune cells-like (CD4+) T cells, (CD14+) monocytes and total number of cells obtained. SVF were isolated from lipoaspirates utilizing a commercially available system (InGeneron Inc.), while MSCs were isolated from SVF via cell culture. Here, we characterized SVF, determined its safety and verify its therapeutic effects in a NOD/scid mouse model of articular injury. However, the heterogeneous cell population within SVF is not historically taken into consideration when injecting into patients.
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Commonly derived from adipose tissue, the stromal vascular fraction (SVF), a heterogeneous cell population enriched with mesenchymal stem cells (MSCs), has garnered interest as a cellular therapy due to ease of accessibility as an autologous, point-of-care application. J Stem Cells Regen Med 2020 16(1) :16-25.doi:10.46582/jsrm.1601004ĭ ue to their capacity to self-renew, proliferate and generate multi-lineage cells, adult-derived stem cells offer great potential in regenerative therapies to treat maladies such as diabetes, cardiac disease, neurological disorders and orthopedic injuries. Human adipose-derived stromal vascular fraction: characterization, safety and therapeutic potential in an experimental mouse model of articular injury. Citation: Dykstra JA, Blue ED, Negrão de Assis PL, Weimer JM, Kota DJ.